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You can vacuum them and it probably won’t kill them initially but as long as the chamber is relatively secure (doesn’t have to be airtight just no obvious way out), they’ll eventually perish via starvation or asphyxiation. I understand that spiders “breathe” through their skin and enough dust can clog that up. Just be prepared not to empty that chamber until you can confirm they’re dead.
You can vacuum them and it probably won’t kill them initially but as long as the chamber is relatively secure (doesn’t have to be airtight just no obvious way out), they’ll eventually perish via starvation or asphyxiation. I understand that spiders “breathe” through their skin and enough dust can clog that up. Just be prepared not to empty that chamber until you can confirm they’re dead.